醫(yī)學全在線
醫(yī)學全在線首頁-醫(yī)師-藥師-護士-衛(wèi)生資格-高級職稱-考試題庫-網(wǎng)校-考研-圖譜-下載-招聘  
分類
國家級省級浙江省各省雜志
科技核心北大核心CSCDCSCD擴展
工具
期刊知識寫作指導 論文投稿推薦期刊
期刊驗證論文檢測 錄用通知往期目錄
SCI
SCI指導影響因子
期刊點評基金動態(tài)
其它
經(jīng)濟教育計算機
建筑體育農(nóng)業(yè)
北京|天津|河北|山西|湖北|江蘇|安徽|山東|上海|浙江|江西|福建|湖南|寧夏|內(nèi)蒙古|河南
四川|重慶|貴州|云南|遼寧|吉林|廣東|廣西|海南|陜西|甘肅|新疆|青海|衛(wèi)生部直屬|黑龍江|兵團
您現(xiàn)在的位置: 醫(yī)學全在線 > 醫(yī)學論文 > 論文投稿 > 正文:醫(yī)學免費論文:基質(zhì)金屬蛋白酶9在哮喘大鼠中性粒細胞中的表達
    

醫(yī)學免費論文:基質(zhì)金屬蛋白酶9在哮喘大鼠中性粒細胞中的表達

來源:本站原創(chuàng) 更新:2013-10-9 論文投稿平臺

醫(yī)學免費論文:基質(zhì)金屬蛋白酶9在哮喘大鼠中性粒細胞中的表達

【摘要】  目的:觀察基質(zhì)金屬蛋白酶9(MMP-9)在哮喘大鼠血中性粒細胞(PMN)中的表達,探討PMN能否通過合成MMP-9參與哮喘的發(fā)病。方法:健康雄性SD大鼠30只,隨機分成哮喘組(A組)、布地奈德治療組(B組)和正常對照組(C組),用卵蛋白(OVA)致敏和激發(fā)的方法建立哮喘模型。分離純化血PMN,免疫細胞化學法檢測MMP-9的表達水平,ELISA法檢測血清基質(zhì)金屬蛋白酶抑制劑-1(TIMP-1)的濃度。結(jié)果:A組(0.196±0.011,OD值)PMN MMP-9的表達水平顯著高于C組(0.100±0.010,OD值)(P <0.01),B組(0.135±0.008,OD值)PMN MMP-9的表達水平顯著低于A組但高于C組(均P <0.01)。A組血清TIMP-1為(34.96±4.54 )ng/mL,表達水平顯著高于C組的(26.14±3.43) ng/mL(P <0.01),B組血清TIMP-1的表達水平顯著低于A組但高于C組(P <0.05或P <0.01)。MMP-9與TIMP-1的表達水平呈顯著正相關(n =29,r=0.713,P <0.01)。結(jié)論:哮喘大鼠PMN合成MMP-9的功能增加,PMN可能部分通過增加合成MMP-9參與哮喘的發(fā)病,這種功能可以部分被布地奈德所抑制。

【關鍵詞】  哮喘;MMP-9;TIMP-1;PMN;大鼠

Expressions of matrix metalloproteinase-9 in blood polymorphonuclear leucocyte of asthmatic rats  JING Wei-ying^,YE Hui,TONG  Xia-sheng,WANG En-zhi,GUO Hai-yuan,RUAN Zheng-ying,JING Xiao-hong.^Department of Pediatrics,Taizhou Integrated Traditional and Western Medicine Hospital,Taizhou, 317523醫(yī).學全.在.線m.52667788.cn

Abstract:  Objective: To observe the expressions of matrix metalloproteinase-9 (MMP-9) in blood polymorphonuclear leukocyte (PMN) of asthmatic rats. And to approach whether PMN could participate in asthma pathogenesis through synthesizing MMP-9. Methods :Thirty rats were randomly divided into three groups on average, including asthmatic group (group A), budesonide treated group (group B) and control group (group C). Blood PMN were isolated and purified. The expressions of MMP-9 were detected with immunocytochemical method in blood PMN. The concentration of matrix metallo-proteinase inhibitor-1 (TIMP-1) was detected with ELISA. Results :The level of MMP-9 in group A (0.196±0.011,Optical density) were significantly higher than those in group C (0.100±0.010)(P <0.01). Levels of MMP-9 in group B (0.135±0.008) were significantly lower than those in group A, but higher than those in group C (all P <0.01). Levels of TIMP-1 in group A (34.96±4.54 ng/mL) were significantly higher than those in group C(26.14±3.43 ng/mL)(P <0.01). Levels of TIMP-1 in group B (29.58±2.41 ng/mL) were significantly lower than those in group A, but higher than those in group C (P <0.05 or P <0.01). There was significant positive correlation between the level of MMP-9 and TIMP-1(n =29,r =0.713,P <0.01). Conclusion:Levels of MMP-9 synthesized by PMN increase in asthma rats. PMN may be implicated with asthma pathogenesis via increasing synthesis of MMP-9,but this function of PMN can be partly inhibited by budesonide.

Key words:  asthma;matrix metalloproteinase-9;TIMP-1;polymorphonuclear leukocyte;rat

支氣管哮喘是一種由多種炎性細胞和細胞組分參與的氣道慢性炎癥,氣道重構(gòu)和氣道高反應性是其特征性病理改變。基質(zhì)金屬蛋白酶9(matrix metalloproteinase-9,MMP-9)及其抑制劑——基質(zhì)金屬蛋白酶抑制劑-1(TIMP-1),在哮喘的氣道炎癥和氣道重構(gòu)中起著重要作用[1]。最近研究發(fā)現(xiàn),中性粒細胞(PMN)能釋放多種酶、炎癥遞質(zhì)和細胞因子參與哮喘的炎癥過程[2],但PMN能否通過MMPs/TIMPs體系參與哮喘的發(fā)病,目前知之甚少。本實驗通過檢測哮喘大鼠血PMN MMP-9和血清TIMP-1的表達水平,探討它們在哮喘中的可能作用機制。


[1] [2] [3] [4] 下一頁

...
關于我們 - 聯(lián)系我們 -版權申明 -誠聘英才 - 網(wǎng)站地圖 - 網(wǎng)絡課程 - 幫助
醫(yī)學全在線 版權所有© CopyRight 2006-2046, MED126.COM, All Rights Reserved
浙ICP備12017320號
百度大聯(lián)盟認證綠色會員實名網(wǎng)站 360認證可信網(wǎng)站 中網(wǎng)驗證